Determinación del estrés oxidativo en caninos de la ciudad de Buenos Aires mediante técnicas analíticas de tipo cualitativo y cuantitativo
Cargando...
Fecha
2023-11-21
Título de la revista
ISSN de la revista
Título del volumen
Editor
Resumen
Las especies reactivas de oxígeno (EROs) son productos del metabolismo celular normal, sus efectos beneficiosos se producen a concentraciones bajas e implican roles fisiológicos importantes. El efecto dañino de los radicales libres se denomina estrés oxidativo y esto ocurre en los sistemas biológicos cuando hay una sobreproducción de EROs y una deficiencia de antioxidantes enzimáticos y no enzimáticos. Los excesos de EROs pueden causar daño celular en lípidos, proteínas o ADN provocando la inhibición de su función normal y generando enfermedades tanto en el ser humano como en los animales. En el presente trabajo se intentó determinar el “estado oxidativo” de caninos clínicamente sanos y enfermos de la ciudad autónoma de Buenos Aires mediante el método cualitativo conocido CMR (capacidad metabólica residual). Asimismo se midieron las concentraciones plasmáticas de malondialdehído (MDA), tioles totales no proteicos y ácido ascórbico con la finalidad de obtener valores cuantitativos con técnicas estandarizadas y evaluarlos en conjunto con los resultados del método CMR.
En los resultados obtenidos en los tres tipos de mediciones de tipo cuantitativo se observó que no hubo diferencias significativas entre los grupos de animales sanos y enfermos, asimismo la interpretación del “estado oxidativo” de todos los valores estimados de CMR fue de desequilibrio redox en ambos grupos de animales. En conclusión, si bien la técnica de CMR posee un buen fundamento teórico y resulta muy práctico para realizar por el profesional debemos ampliar los estudios para validar esta técnica de análisis.
- Reactive oxygen species (ROS) are products of normal cellular metabolism, their beneficial effects occur at low or moderate concentrations, and they play important physiological roles. The damaging effect of free radicals is called oxidative stress and this occurs in biological systems when there is an overproduction of ROS and a deficiency of enzymatic and non-enzymatic antioxidants. ROS excesses can cause cellular damage to lipids, proteins or DNA, causing the inhibition of their normal function and generating diseases in both humans and animals. In the present work, an attempt was made to determine the "oxidative state" of clinically healthy and diseased canines from Buenos Aires City by means of the qualitative method known as RMC (residual metabolic capacity). Plasma concentrations of malondialdehyde (MDA), total non-protein thiols and ascorbic acid were also measured in order to obtain quantitative values with standardized techniques and evaluate them together with the results of the RMC method. In the results obtained in the three types of quantitative measurements, it was observed that there were no significant differences between the groups of healthy and sick animals, likewise the interpretation of the "oxidative state" of all the estimated values of RMC was of redox imbalance in both. animal groups. In conclusion, although the RMC technique has a good theoretical foundation and is very practical to be carried out by the professional, we must expand the studies to validate this analysis technique.
- Reactive oxygen species (ROS) are products of normal cellular metabolism, their beneficial effects occur at low or moderate concentrations, and they play important physiological roles. The damaging effect of free radicals is called oxidative stress and this occurs in biological systems when there is an overproduction of ROS and a deficiency of enzymatic and non-enzymatic antioxidants. ROS excesses can cause cellular damage to lipids, proteins or DNA, causing the inhibition of their normal function and generating diseases in both humans and animals. In the present work, an attempt was made to determine the "oxidative state" of clinically healthy and diseased canines from Buenos Aires City by means of the qualitative method known as RMC (residual metabolic capacity). Plasma concentrations of malondialdehyde (MDA), total non-protein thiols and ascorbic acid were also measured in order to obtain quantitative values with standardized techniques and evaluate them together with the results of the RMC method. In the results obtained in the three types of quantitative measurements, it was observed that there were no significant differences between the groups of healthy and sick animals, likewise the interpretation of the "oxidative state" of all the estimated values of RMC was of redox imbalance in both. animal groups. In conclusion, although the RMC technique has a good theoretical foundation and is very practical to be carried out by the professional, we must expand the studies to validate this analysis technique.
Descripción
Palabras clave
Estrés oxidativo, Antioxidantes, CMR, Técnicas, Oxidative stress, Antioxidants, RMC, Techniques
Citación
Meneses, M. L., La Valle, J. y Recalde, M. (2023). Determinación del estrés oxidativo en caninos de la ciudad de Buenos Aires mediante técnicas analíticas de tipo cualitativo y cuantitativo. Contribuciones de Ciencia y Tecnología, 1(1). https://rid.unaj.edu.ar/handle/123456789/2805